Cd27 Antibody

• Catalog Number: orb1671910
• Category: Antibodies
• Description: Cd27 Antibody
• Clonality: Recombinant
• Clone Number: LG.3A10
• Tested applications: Activation, FC, IF, IP
• Reactivity: Human, Mouse, Rat
• Isotype: IgG kappa
• Immunogen: This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Cd27
• UniProt ID: P41272
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRS7, CD27L receptor, T-cell activation antigen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of LG.3A10 (orb1671910) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671910- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.

Binding curve of anti-CD27 antibody LG.3A10 (orb1671910) to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using orb1671910. For detection- a 1:4000 dilution of HRP-loat anti-ribody was used.