CD255 antibody (PE)

• Catalog Number: orb222756
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD255 conjugated to PE
• Clonality: Monoclonal
• Clone Number: CARL-1
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG3 kappa
• Immunogen: human CD255-transfected 2PK-3 cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD255
• Entrez: 8742
• UniProt ID: O43508
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: TWEAK
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of CD255 transfected L5178Y cells (red-filled) from nontransfected L5178Y cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD255 (CARL-1) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).