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Catalog Number | orb1973145 |
---|---|
Category | Antibodies |
Description | CD25 / IL-2R alpha antibody [M431] |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human |
Dilution range | Western Blot 1:500 Immunofluorescence 1:200 – 1:300 |
Conjugation | Unconjugated |
Target | CD25 / IL-2R alpha antibody [M431] |
Storage | Long time storage is recommended at -20°C Phosphate-buffered solutions, pH 7.2, 50% Glycerol, 0.025% ProClin 300 was added as a preservative Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1973145 overnight at 4°C. Lysates at 30 μg per lane. Anti-CD25/IL-2R alpha antibody [M431] at 1/500 dilution.
Immunohistochemical analysis of paraffin embedded Human tonsil cancer tissue labeling CD25/IL-2R alpha antibody with orb1973145 at 1/200.
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