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CD25 Antibody / IL2RA

Catalog Number: orb2644485

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2644485
CategoryAntibodies
DescriptionRecognizes a protein of 55kDa, identified as CD25 (Workshop IV; Code A27). CD25 is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25.
Species/HostMouse
ClonalityMonoclonal
Clone NumberIL2RA/2393
Tested applicationsELISA
ReactivityHuman
IsotypeMouse IgG2b, kappa
ImmunogenStimulated human leukocytes were used as the immunogen for the CD25 antibody.
Antibody TypePrimary Antibody
Dilution rangeELISA: order Ab without BSA for coating
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis CD25 antibody is available for research use only.
UniProt IDP01589
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the CD25 antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
CD25 Antibody / IL2RA

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD25 antibody (clone IL2RA/2393). These results demonstrate the foremost specificity of the IL2RA/2393 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

CD25 Antibody / IL2RA

SDS-PAGE analysis of purified, BSA-free CD25 antibody (clone IL2RA/2393) as confirmation of integrity and purity.

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