CD25 Antibody / IL2RA

• Catalog Number: orb1826310
• Category: Antibodies
• Description: CD25, or interleukin 2 (IL2) receptor alpha (IL2RA), and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (CD25) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolysis. CD25 (the p55 chain of the interleukin-2 receptor) is a marker of activation for a number of cell types, including T cells, B cells, and macrophages. It also appears to be a reliable immunohistochemical marker for the discrimination of neoplastic from normal/reactive mast cells, with potential as a diagnostic tool in systemic mastocytosis. CD25 is also expressed on hairy cell leukemia.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein (within amino acids 1-250) from the human protein was used as the immunogen for the CD25 antibody.
• UniProt ID: P01589
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Antibody Type: Primary Antibody
• Clone Number: IL2RA/4375R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the CD25 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This CD25 antibody is available for research use only.
• Note: For research use only

SDS-PAGE analysis of purified, BSA-free CD25 antibody (clone IL2RA/4375R) as confirmation of integrity and purity.