CD25 antibody (FITC)

• Catalog Number: orb44157
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD25 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: MEM-181
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: PHA-activated peripheral blood leucocytes
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD25
• Entrez: 3559
• UniProt ID: P01589
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD25 antibody (FITC), anti CD 25 antibody (FI
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human PBMC with anti-human CD25 (MEM-181) FITC. The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C - staining with anti-human CD25 (MEM-181); Panel B, D - staining with a standard anti-CD25 monoclonal antibody.