CD25 antibody (APC)

• Catalog Number: orb198253
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD25 conjugated to APC
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ITYV
• Tested applications: FACS
• Reactivity: Human
• Isotype: IgG1
• Immunogen: CD25=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with PHA-activated human T-lymphocytes.
• Concentration: Titered for flow cytometry
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Conjugation: APC
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P01589
• Storage: Product should be stored at 4-8°C. DO NOT FREEZE
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Alternative names: CD25*APC
• Note: For research use only
• Application notes: PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Peripheral blood lymphocytes using CD25 antibody (APC)