CD23/FCER2 Antibody

• Catalog Number: orb182383
• Category: Antibodies
• Description: CD23/FCER2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry, 0.5-1μg/ml, Human Immunofluorescence, 2μg/ml, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Mouse ELISA (Cap), 1-5μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 37648 MW
• UniProt ID: P20693
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Low affinity immunoglobulin epsilon Fc receptor;Fc
• Note: For research use only
• Application notes: WB: The detection limit for CD23 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of mouse PBMC cells using anti-CD23 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of mouse BMC cells using anti-CD23 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD23 using anti-CD23 antibody.Lane 1:Mouse Liver Tissue.

WB analysis of CD23 using anti-CD23 antibody.Lane 1:rat spleen tissue;2:rat thymus tissue.

IF analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of mouse lymphaden tissues.

IF analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of rat lymphaden tissues.

Sandwich ELISA - Recombinant mouse CD23/FCER2 protein standard curve.Use in combination with reagents from Mouse CD23/FCER2 ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Human Tonsil Tissue.

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Mouse Spleen Tissue.

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Rat Spleen Tissue.

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in frozen section of mouse spleen tissues.