Cd226 Antibody

• Catalog Number: orb1671694
• Category: Antibodies
• Description: Cd226 Antibody
• Clonality: Recombinant
• Clone Number: 10E5
• Tested applications: FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising Lewis and Lou/M rats with the murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice, before screening for reactivity with Th1 clones and a lack of reactivity to Th2/Th0 cell lines.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Cd226
• UniProt ID: Q8K4F0
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: DNAM-1, PTA1, PTA-1, platelet and T cell activatio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-CD266 antibody 10E5. Mouse spleen tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of 10E5 (orb1671694) at 2 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the Anti-CD226 antibody 10E5. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 10E5 (orb1671694 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.