CD22 Antibody (monoclonal, 5E7)

• Catalog Number: orb654262
• Category: Antibodies
• Description: CD22 Antibody (monoclonal, 5E7)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5E7
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (696-724aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.
• Concentration: 0
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 140 kDa
• UniProt ID: P20273
• Sensitivity: > 5000 cells
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: B-cell receptor CD22; B-lymphocyte cell adhesion m
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD22 using anti ZO-1 antibody (orb654262). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD22 antigen affinity purified polyclonal antibody (Catalog # orb654262) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for CD22 at approximately 140KD. The expected band size for CD22is at 95KD.

Flow Cytometry analysis of PBMC cells using anti-CD22 antibody (orb654262). Overlay histogram showing PBMC cells stained with orb654262 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD22 Antibody (orb654262, 1?g/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10?g/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1?g/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.