CD207 Antibody (PE)

• Catalog Number: orb1993740
• Category: Antibodies
• Description: CD207 is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway.
• Clonality: Monoclonal
• Clone Number: 2G3
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 lambda
• Immunogen: Fusion protein of human CD207 extracellular part and IgG Fc fragment
• Conjugation: PE
• Target: CD207
• Entrez: 50489
• UniProt ID: Q9UJ71
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: CLEC4K, langerin
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Separation of human CD207 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).