CD205 antibody (PE )

• Catalog Number: orb154513
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PE which recognizes CD205 that also known as DEC-205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity.
• Clonality: Monoclonal
• Clone Number: HD30
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant Fc-tagged human CD205
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD205
• Entrez: 4065
• UniProt ID: O60449
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-DEC-205 antibody, anti-LY75 antibody, anti-CL
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD205 (HD30) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human HLA-DR (MEM-12) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human monocytes (red-filled) from CD205 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD205 (HD30) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD205 (HD30) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).