CD203c antibody (APC)

• Catalog Number: orb154435
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to APC which recognizes CD203c that expression increases in response to a variety of allergens, including cat dander, latex, and bee and wasp venom. CD203c upregulation may also serve as a tumor marker for colon carcinoma. It is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching,
• Clonality: Monoclonal
• Clone Number: NP4D6
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: HEK-293 cells transfected with human CD203c
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD203c
• Entrez: 5169
• UniProt ID: O14638
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-ENPP3 antibody, anti-B10 antibody, anti-PDNP3
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human basophils in IgE-activated whole blood by anti-CD203c antibody (NP4D6) APC.

Flow cytometry multicolor staining pattern of human IgE-stimulated PBMC stained using anti-human CD107a (H4A3) Pacific Blue™ and anti-human CD203c (NP4D6) APC.