CD2 Antibody

• Catalog Number: orb2637559
• Category: Antibodies
• Description: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1E7E8.G4
• Tested applications: FACS, Functional Studies, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Human Thymocytes/Sezary T cells were used as the immunogen for this CD2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD2 antibody is available for research use only.
• Entrez: 914
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Jurkat cell lysate with CD2 antibody (clone 1E7E8.G4). Expected molecular weight: 38-50 kDa depending on level of glycosylation.

Immunofluorescent staining of human Molt-4 cells with CD2 antibody (clone 1E7E8.G4, green) and Reddot nuclear stain (red).

Flow cytometry staining of human Molt-4 cells with CD2 antibody (clone 1E7E8.G4); Red = isotype control, Blue = CD2 antibody.

SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone 1E7E8.G4) as confirmation of integrity and purity.