CD2 antibody (APC)

• Catalog Number: orb198246
• Category: Antibodies
• Description: Mouse Monoclonal antibody to CD2 (APC)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: T6.3
• Tested applications: FACS
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture.
• Concentration: Titered for flow cytometry
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Conjugation: APC
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P06729
• Storage: Product should be stored at 4-8°C. DO NOT FREEZE
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Alternative names: T-cell surface antigen CD2; CD2 cytoplasmic domain
• Note: For research use only
• Application notes: PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. . See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Peripheral blood lymphocytes using CD2 antibody (APC)