CD1d Antibody (PE)

• Catalog Number: orb1676212
• Category: Antibodies
• Description: CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.
• Clonality: Monoclonal
• Clone Number: 51.1
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG2b kappa
• Immunogen: human CD1d
• Conjugation: PE
• Target: CD1d
• Entrez: 912
• UniProt ID: P15813
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human gated lymphocytes stained using anti-human CD1d (51.1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD1d positive CD19 positive lymphocytes (red-filled) from CD1d negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD1d (51.1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).