CD1b antibody (FITC)

• Catalog Number: orb179864
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to FITC which recognizes CD1b.
• Clonality: Monoclonal
• Clone Number: SN13
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: A cell membrane antigen preparation that was isolated from normal human thymocytes
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD1b
• Entrez: 910
• UniProt ID: P29016
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-R1 antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human CD1b positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red) from CD1b negative monocytes (blue) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).