CD19 antibody (Biotin)

• Catalog Number: orb154467
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to Biotin which recognizes CD19 that amplifies the signaling cascade in B cells. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK).CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells.Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3).
• Clonality: Monoclonal
• Clone Number: 4G7
• Tested applications: FC, ICC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Human CCL (chronic lymphocytic leukemia) cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytrometry: Recommended dilution: 1 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD19
• Entrez: 930
• UniProt ID: P15391
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-B4 antibody, anti-Leu-12 antibody, anti-CVID3
• Note: For research use only
• Application notes: Flow cytrometry: Recommended dilution: 1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human peripheral blood leukocytes with anti-human CD19 (4G7) biotin / streptavidin-PE.