CD18/ITGB2 Antibody

• Catalog Number: orb215895
• Category: Antibodies
• Description: CD18/ITGB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Immunofluorescence, 5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 84782 MW
• UniProt ID: P05107
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Integrin beta-2;Cell surface adhesion glycoprotein
• Note: For research use only
• Application notes: WB: The detection limit for CD18 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody.Lane 1:human Jurkat cell;2:human HeLa cell.

IF analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody. CD18/ITGB2 was detected in a paraffin-embedded section of human tonsil tissue.

IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody. CD18/ITGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody. CD18/ITGB2 was detected in a paraffin-embedded section of human lung cancer tissue.