CD18 antibody

• Catalog Number: orb759015
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD18
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 60.3
• Tested applications: Blocking, ELISA, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD18
• UniProt ID: P05107
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ITGB2; MFI7; Integrin beta-2; Cell surface adhesio
• Note: For research use only
• Application notes: This antibody is capable of inhibiting several cell mediated immune functions. It inhibits proliferative responses of T cells stimulated by soluble antigens, mitogens, or allogeneic cells in the initiation of a culture, but not when added after 48 hours. It successfully blocks the lytic activity of cytotoxic T cells and natural killer cells and the migration of neutrophilic granulocytes (PMID: 6644024). This antibody inhibits phagocytosis by reaction with the C3bi receptor, and stimulates phagocytosis by reverse opsonization (PMID: 3880787). This antibody inhibits aggregation of neutrophils in response phorbol myristate acetate, zymosan-activated plasma, and N-formyl-methionylleucylphenylalanine in a dose dependent manner (PMID: 3995185). 60.3 also inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo (PMID: 3539230). 60.3 successfully inhibits neutrophil function in vivo and has the potential to be used as an anti-inflammatory agent (PMID: 3693901). This antibody has application in blocking neutrophil mediated organ damage which occurs after myocardial infarct or hemorrhagic shock. Humanized version of this antibody binds CD18 with a 4-fold less affinity than its parent murine version (PMID: 7937713).
• Expiration Date: 12 months from date of receipt.