CD18 antibody

• Catalog Number: orb44079
• Category: Antibodies
• Description: Mouse Monoclonal to CD18.
• Clonality: Monoclonal
• Clone Number: MEM-148
• Tested applications: FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Peripheral blood mononuclear cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 8 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD18
• Entrez: 3689
• UniProt ID: P05107
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD18 antibody, anti-Integrin beta2 subunit an
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 8 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of PMA-activated neutrophils (A) and monocytes (B), using anti-human CD18 (MEM-148). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

Separation of human monocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 µg/ml) GAM APC.