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Catalog Number | orb348903 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to CD16/CD32 |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | 2.4G2 |
Tested applications | Blocking, FC, IF, IHC, IP |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Mouse BALB/c Macrophage J774 Cell Line. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | CD16/CD32 |
UniProt ID | P08101, P08508 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Fcγ III/II Receptor; Low affinity immunoglobulin g Read more... |
Note | For research use only |
Application notes | This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies made by Biorbyt that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors. |
Expiration Date | 12 months from date of receipt. |
Unkeless, J. C. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors J Exp Med, 150, 580-596 (1979)
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