CD166/ALCAM Antibody

• Catalog Number: orb18529
• Category: Antibodies
• Description: CD166/ALCAM Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CD166/ALCAM recombinant protein (Position: N167-E406). Human CD166/ALCAM shares 90.8% amino acid (aa) sequence identity with both mouse and rat CD166/ALCAM.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 65102 MW
• UniProt ID: Q13740
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CD166 antigen;Activated leukocyte cell adhesion mo
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SH-SY5Y cells using anti-CD166/ALCAM antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CD166/ALCAM using anti-CD166/ALCAM antibody.Lane 1:human SH-SY5Y cell; 2:human HeLa cell; 3:rat liver tissue; 4:rat brain tissue; 5:rat lung tissue; 6:mouse liver tissue; 7:mouse brain tissue; 8:mouse lung tissue.

IF analysis of CD166/ALCAM using anti-CD166/ALCAM antibody. CD166/ALCAM was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of CD166/ALCAM using anti-CD166/ALCAM antibody. CD166/ALCAM was detected in a paraffin-embedded section of human breast cancer tissue.

IHC analysis of CD166/ALCAM using anti-CD166/ALCAM antibody. CD166/ALCAM was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of CD166/ALCAM using anti-CD166/ALCAM antibody. CD166/ALCAM was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of CD166/ALCAM using anti-CD166/ALCAM antibody. CD166/ALCAM was detected in a paraffin-embedded section of mouse lung tissue.

IHC analysis of CD166/ALCAM using anti-CD166/ALCAM antibody. CD166/ALCAM was detected in a paraffin-embedded section of rat lung tissue.

IHC analysis of CD166/ALCAM using anti-CD166/ALCAM antibody. CD166/ALCAM was detected in a paraffin-embedded section of rat lung tissue.