CD163 antibody (APC)

• Catalog Number: orb154443
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to APC which recognizes CD163 that also designated M130 is a macrophage-associated antigen that is a member of the scavenger receptor cysteine-rich superfamily. CD163 is a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin- hemoglobin complexes. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme.
• Clonality: Monoclonal
• Clone Number: GHI/61
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Hairy cell leukemia cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD163
• Entrez: 9332
• UniProt ID: Q86VB7
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-MM130 antibody, anti-SCARI1 antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human peripheral blood using anti-human CD163 (clone GHI/61) APC. CD163 was detected on CD14+ monocytes, whereas CD14- lymphocytes were used as a negative control.