CD16 antibody (PE)

• Catalog Number: orb44656
• Category: Antibodies
• Description: Mouse Monoclonal to CD16.
• Clonality: Monoclonal
• Clone Number: 3G8
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Human neutrophils
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD16
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD16 antibody, anti-FACSgammaRIII antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD16 (3G8) PE antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 negative CD16 positive NK cells (red-filled) from human CD3 positive CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (3G8) PE (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).