CD16 antibody (FITC)

• Catalog Number: orb623582
• Category: Antibodies
• Description: Rat monoclonal antibody against CD16 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: 93
• Tested applications: FC
• Reactivity: Mouse
• Isotype: Rat IgG2a lambda
• Immunogen: Murine pre-B cells
• Concentration: 0.5 mg/ml
• Dilution range: FACS: 2-5 μg/ml
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD16/CD32
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD16 antibody, anti-CD32 antibody, anti-Fcgam
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of murine CD16/32 positive cells (red-filled) from murine CD16/32 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 µg/ml).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 µg/ml).