CD16 antibody (Biotin)

• Catalog Number: orb44103
• Category: Antibodies
• Description: Mouse Monoclonal to CD16.
• Clonality: Monoclonal
• Clone Number: MEM-154
• Tested applications: FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human granulocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 5-8 μg/ml; positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD16
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD16 antibody, anti-FACSgammaRIII antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 5-8 μg/ml; positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
• Expiration Date: 12 months from date of receipt.

Separation of neutrophil granulocytes stained anti-human CD16 (MEM-154) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC, red-filled) from neutrophil granulocytes unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (MEM-154) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC).