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Catalog Number | orb154525 |
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Category | Antibodies |
Description | Mouse monoclonal antibody conjugated to PE which recognizes CD148 that is also known as DEP-1, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. It is widely expressed in tissues, particularly those forming epithelioid monolayers. CD148 can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. |
Clonality | Monoclonal |
Clone Number | MEM-CD148/05 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG2b |
Immunogen | Human recombinant CD148 (amino acids 1-444) |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | CD148 |
Entrez | 5795 |
UniProt ID | Q12913 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-HPTP-eta antibody, anti-DEP-1 antibody, anti- Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis (surface staining) of human peripheral blood leukocytes using anti-CD148 (MEM-CD148/05) PE.
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