CD147/Emmprin/BSG Antibody

• Catalog Number: orb389463
• Category: Antibodies
• Description: CD147/Emmprin/BSG Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CD147/Emmprin recombinant protein (Position: E138-A323). Human CD147/Emmprin shares 51.1% and 51.9% amino acid (aa) sequence identity with mouse and rat CD147/Emmprin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 42200 MW
• UniProt ID: P35613
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Basigin;5F7;Collagenase stimulatory factor;Extrace
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HeLa cells using anti-CD147/Emmprin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A549 cells using anti-CD147/Emmprin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD147/Emmprin using anti-CD147/Emmprin antibody.Lane 1:human HeLa cell; 2:human HepG2 cell.

IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody.CD147/Emmprin was detected in immunocytochemical section of A549 cell.

IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody.CD147/Emmprin was detected in immunocytochemical section of A549 cell.

IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody.CD147/Emmprin was detected in immunocytochemical section of HELA cell.

IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody.CD147/Emmprin was detected in paraffin-embedded section of human intestinal cancer tissues.

Sandwich ELISA - Recombinant human Emmprin protein standard curve.Use in combination with reagents from Human Emmprin ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in paraffin-embedded section of human tonsil tissues.