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Catalog Number | orb1956992 |
---|---|
Category | Proteins |
Description | CD14 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.5 kDa and the accession number is P08571. |
Tag | C-His |
Purity | SDS-PAGE: 96.9%; SEC-HPLC: 89.5% |
MW | 36.5 kDa (predicted); 45 kDa (reducing condition, due to glycosylation) |
UniProt ID | P08571 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | CD14 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.5 kDa and the accession number is P08571. |
Expression Region | A DNA sequence encoding the mature form of human CD14 (NP_000582.1) (Met 1-Met 344) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Thr 20 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 36.7 kDa after removal of the signal peptide. The apparent molecular mass of CD14(20-352)-7xHis is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 35.9 kDa after removal of the signal peptide. The apparent molecular mass of CD14(20-344)-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
98.00% | |
45 KDa (reducing condition) |
94.00% | |
55 KDa (reducing condition) |