CD14 (Monocyte / Macrophage Marker) Antibody

• Catalog Number: orb2316531
• Category: Antibodies
• Description: CD14 (Monocyte / Macrophage Marker) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LPSR/2397
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 55kDa
• Target: CD14
• Entrez: 929
• UniProt ID: P08571
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Lipopolysaccharide receptor; LPS-R; LPSR; Mo2; Mon
• Note: For research use only
• Application notes: Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
• Expiration Date: 12 months from date of receipt.