CD14 Antibody (Biotin)

• Catalog Number: orb1671422
• Category: Antibodies
• Description: CD14 Antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human CD14-specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: CD14
• UniProt ID: P08571
• NCBI: P08571
• Storage: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: soluble CD14, Monocyte differentiation antigen CD1
• Note: For research use only
• Application notes: Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human sCD14 as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sCD14. Western Blot To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human sCD14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sCD14 as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sCD14.

To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.