CD135 / FLT3 Antibody (Internal)

• Catalog Number: orb1534432
• Category: Antibodies
• Description: CD135 / FLT3 Antibody (Internal)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P
• Reactivity: Human
• Immunogen: Synthetic 20 amino acid peptide from internal region of human FLT3 / CD135. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Rabbit (95%); Hamster (85%); Bovine, Dog, Panda, Horse (80%).
• Concentration: 1 mg/ml
• Dilution range: ELISA, IHC, IHC-P (8 µg/ml)
• Conjugation: Unconjugated
• Target: CD135 / FLT3
• Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide
• Alternative names: FLT3, CD135, CD135 antigen, Fetal liver kinase 2,
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 8 ug/ml.
• Expiration Date: 12 months from date of receipt.