CD135 antibody (APC)

• Catalog Number: orb545659
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD135 conjugated to APC
• Clonality: Monoclonal
• Clone Number: BV10A4
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: BV-173 leukemic cell line
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD135
• Entrez: 2322
• UniProt ID: P36888
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-FLT3 antibody, anti-FLK2 antibody, anti-STK1
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of REH cells stained using anti-human CD135 (BV10A4) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from REH cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as CD135 APC concentration, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of REH cell suspension stained using anti-human CD135 (BV10A4) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension).