CD134 antibody (FITC)

• Catalog Number: orb421967
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD134 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: Ber-ACT35
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: HTLV 1-transformed HUT-102 cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD134
• Entrez: 7293
• UniProt ID: P43489
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TNFRSF4 antibody, anti-OX40 antibody, anti-AC
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD25 (MEM-181) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD134 positive CD25 positive cells (red-filled) from CD134 negative CD25 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).