CD133 antibody (PE)

• Catalog Number: orb545657
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD133 conjugated to PE
• Clonality: Monoclonal
• Clone Number: 293C3
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: human CD133 transfectants
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD133
• Entrez: 8842
• UniProt ID: O43490
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Prominin 1 antibody, anti-PROM1 antibody, ant
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of CD133 in human peripheral blood with anti-CD133 (293C3) PE.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD133 (293C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD34 (581) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD34 positive cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD133 (293C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD133 (293C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).