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Catalog Number | orb222744 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD129 conjugated to PE |
Clonality | Monoclonal |
Clone Number | AH9R7 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG2b kappa |
Immunogen | Human CD129-transfected cell line |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | CD129 |
Entrez | 3581 |
UniProt ID | Q01113 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | IL-9R alpha Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of HuT-78 cells stained using anti-human CD129 (AH9R7) PE antibody (concentration in sample 9 µg/ml, red) from unstained HuT-78 cells (grey) in flow cytometry analysis (surface staining).
Separation of HUT-78 cells stained using anti-human CD129 (AH9R7) PE antibody (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from HUT-78 cells stained using mouse IgG2b isotype control (MPC-11) PE antibody (concentration in sample 5 µg/ml, same as CD129 PE antibody concentration, black-dashed) in flow cytometry analysis (surface staining).
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