CD123 Antibody (PerCP)

• Catalog Number: orb1742779
• Category: Antibodies
• Description: CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
• Clonality: Monoclonal
• Clone Number: 6H6
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
• Conjugation: PerCP
• Target: CD123
• Entrez: 3563
• UniProt ID: P26951
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: IL3RA, MGC34174, hIL-3Ra
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human mononuclear cells stained using anti-human CD123 (6H6) PerCP antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD123 positive CD45dim leukocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD123 (6H6) PerCP antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) PerCP antibody (10 μl reagent / 100 μl of peripheral whole blood).