CD123 antibody

• Catalog Number: orb1152465
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD123
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7G3
• Tested applications: Blocking, FC, IP, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: The original antibody was generated by intraperitoneally immunizing BALB/c mice with 5 X lo6 COS cells transfected with the IL-3R a-chain in combination with adjuvant peptides.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD123
• UniProt ID: P26951
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IL3RA; IL-3RA; IL-3R-alpha; Interleukin-3 receptor
• Note: For research use only
• Application notes: This antagonist antibody binds IL-3Rα and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R α chain expressed by transfected cells and bound to primary cells expressing IL-3Rα. The expression of IL-3Rα chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Rα and βc dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38− cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcγRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514).
• Expiration Date: 12 months from date of receipt.