CD11b antibody

• Catalog Number: orb665642
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD11b
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD11b. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ITGAM
• Entrez: 3684
• UniProt ID: P11215
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-CD11B antibody, anti-CR3A antibody, anti-Inte
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD11b expression in SHSY5Y (A), U87MG (B), A549 (C) whole cell lysates. (Predicted band size: 127 kD; Observed band size: 170 kD)

Immunohistochemical analysis of CD11b staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.