CD11b antibody

• Catalog Number: orb44135
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD11b
• Clonality: Monoclonal
• Clone Number: MEM-174
• Tested applications: FC, IP
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Human granulocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes. Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants. Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD11b
• Entrez: 3684
• UniProt ID: P11215
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti Antigen CD11b p170 antibody, anti CD11 antige
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes. Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants. Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. Lysate was subjected to affinity column chromatography using anti-human CD11b (MEM-174) immunosorbent. Eluted immunoprecipitate was resolved on 7.5% SDS-PAGE and stained with Coomasie Blue.

Separation of human monocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11b (MEM-174) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (MEM-174) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).