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    CD11b antibody

    Catalog Number: orb248883

    DispatchUsually dispatched within 5-10 working days
    $ 267.00
    Catalog Numberorb248883
    CategoryAntibodies
    DescriptionMouse monoclonal antibody to CD11b
    Species/HostRat
    ClonalityMonoclonal
    Clone NumberM1/70
    Tested applicationsFC, IF
    ReactivityHuman
    ImmunogenNative purified human CD11b.
    Form/AppearanceRat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
    ConjugationUnconjugated
    TargetITGAM
    Entrez3684
    UniProt IDP11215
    SourceRat
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesRat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
    Alternative namesanti Antigen CD11b p170 antibody, anti CD11 antige
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    NoteFor research use only
    Application notes1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    Expiration Date12 months from date of receipt.
    CD11b antibody

    Flow cytometric analysis of human peripheral blood granulocytes using Anti-CD11b Antibody, followed by anti-mouse IgG PE.

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