CD11b antibody (Biotin)

• Catalog Number: orb44137
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD11b conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: MEM-174
• Tested applications: FC, IP
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Human granulocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD11b
• Entrez: 3684
• UniProt ID: P11215
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti Antigen CD11b p170 antibody (Biotin), anti CD
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes.
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11b (MEM-174) Biotin antibody (concentration in sample 6 µg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (MEM-174) Biotin antibody (concentration in sample 6 µg/ml, Streptavidin APC).