CD117 (pY936) antibody

• Catalog Number: orb665478
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD117 (Phospho-Y936)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Porcine
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y936 of human CD117 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: KIT
• Entrez: 3815
• UniProt ID: P10721
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-SCFR antibody, anti-Mast/stem cell growth fac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD117 (Phospho-Y936) expression in A375 (A), SGC7901 (B), A2780 (C) whole cell lysates. (Predicted band size: 109 kD; Observed band size: 145 kD)

Immunohistochemical analysis of CD117 (Phospho-Y936) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.