CD107b antibody

• Catalog Number: orb44718
• Category: Antibodies
• Description: Mouse Monoclonal to CD107b.
• Clonality: Monoclonal
• Clone Number: H4B4
• Tested applications: FC, ICC, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Human PBMC
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Intracellular and extracellular staining; recommended dilution: 1-2 μg/ml. Immunohistochemistry: Recommended dilution: 2-8 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD107b
• Entrez: 3920
• UniProt ID: P13473
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-CD107b antibody, anti-LAMP-2 antibody
• Note: For research use only
• Application notes: Flow cytometry: Intracellular and extracellular staining; recommended dilution: 1-2 μg/ml. Immunohistochemistry: Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human anti-IgE antibody stimulated mononuclear cells stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) and anti-human CD203c (NP4D6) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD107b positive CD203c positive basophil granulocytes (red-filled) from CD107b negative CD203c positive basophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human anti-IgE antibody stimulated peripheral whole blood stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human anti-IgE antibody stimulated peripheral whole blood stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Anti-Hu CD107b Purified (clone H4B4) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of SK-MEL30, MCF-7, THP-1, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody H4B4 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed. CD107b molecules were detected at ~95-125 kDa in all tested cell lines under both reducing and non-reducing conditions.