CD105 antibody (Biotin)

• Catalog Number: orb154464
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to Biotin which recognizes CD105 that also known as Endoglin, is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1). It is a major glycoprotein of vascular endothelium. It may play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
• Clonality: Monoclonal
• Clone Number: MEM-229
• Tested applications: FC, ICC, IHC-Fr, WB
• Reactivity: Human, Porcine
• Isotype: Mouse IgG2a
• Immunogen: Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 8 μg/ml; positive control: Kg1 human acute myelogenous leukemia cell line.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD105
• Entrez: 2022
• UniProt ID: P17813
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Endoglin antibody, anti-END antibody, anti-EN
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 8 μg/ml; positive control: Kg1 human acute myelogenous leukemia cell line.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of CD105 on Huvec and HEK-293 cells with anti-CD105 (MEM-229) biotin.