CCT5 Antibody

• Catalog Number: orb1524756
• Category: Antibodies
• Description: CCT5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 100 and 150
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CCT5
• UniProt ID: P48643
• NCBI: NP_036205.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HEL-S-69; TCP-1-epsilon; T-complex protein 1 subun
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse CCT5 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) , HeLa (H; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CCT5 antibody orb1524756 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. CCT5 was also immunoprecipitated by rabbit anti-CCT5 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Detection of mouse CCT5 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CCT5 (Cat. No. orb1524756) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human CCT5 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CCT5 (Cat. No. orb1524756) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).