CCRL2 Antibody [BZ5B8], Rabbit IgG

• Catalog Number: orb1671782
• Category: Antibodies
• Description: CCRL2 Antibody [BZ5B8], Rabbit IgG
• Clonality: Recombinant
• Clone Number: BZ5B8
• Tested applications: ELISA, FA, FC, ICC, IHC, IP, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1–24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Ccrl2
• UniProt ID: O35457
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CCR11, C-C chemokine receptor-like 2, Chemokine re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CCRL2 antibody BZ5B8 (B467). Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of BZ5B8 (orb1671782) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671782- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CCRL2 antibody. Mouse thymus (A) and mouse brain (B) tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of CCRL2 (orb1671782) at 0.5 ug/ml- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the anti-CCRL2 antibody BZ5B8. RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of BZ5B8 (orb1671782 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.