Ccr9 Antibody

• Catalog Number: orb1671594
• Category: Antibodies
• Description: Ccr9 Antibody
• Clonality: Recombinant
• Clone Number: 9B1
• Tested applications: FC, NeA
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing rats with KLH-conjugated linear peptide corresponding to the N-terminus of mouse CCR9.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Ccr9
• UniProt ID: Q9WUT7
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD199, C-C chemokine receptor type 9, C-C CKR-9, C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CCR9 antibody 9B1. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 9B1 (orb1671594 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CCR9 antibody 9B1. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of 9B1 (orb1671594) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671594- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.