CCR7 antibody

• Catalog Number: orb537278
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CCR7
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 330-372 of human CCR7 (NP_001829.1).
• Dilution range: WB: 1:500-1:2000
• Conjugation: Unconjugated
• MW: 45kDa
• UniProt ID: P32248
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: CCR7, BLR2, CC-CKR-7, CCR-7, CD197, CDw197, CMKBR7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of RAW264.7 cells using CCR7 Rabbit pAb (orb537278) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of lysates from A-549 cells, using CCR7 Rabbit pAb (orb537278) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of lysates from Mouse lung, using CCR7 Rabbit pAb (orb537278) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of lysates from Rat lung, using CCR7 Rabbit pAb (orb537278) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.